Development and Validation of Rp-hplc Method for the Estimation of Strontium Ranelate in Sachet K.mythili, S.gayatri, Koya Ravi Teja, K.chitra and C.uma

A rapid and sensitive RP-HPLC method with UV detection (323 nm) for routine analysis of strontium ranelate in bulk and in pharmaceutical formulation as sachet was developed. Chromatography was performed with mobile phase containing a mixture of monobasic potassium phosphate buffer and methanol (3:1 v/v) with the flow rate of 0.8 ml/min at 25 o C. In the range of 50-250 μg/ml, the linearity of strontium ranelate shows a correlation coefficient of 0.999. The percentage recovery studies were found to be in the range of 97-102 %. The proposed method was validated by determining accuracy, precision and ruggedness. The results of all the validation parameters were well within their acceptance values.